| Microorganism name |
Kluyveromyces lactis
|
| Target proteins |
Brazzein (Park et al., 2021)
|
| Wild-type or GMO |
GMO (Park et al., 2021)
|
| Production mode (intracellular/extracellular) |
Extracellular (Park et al., 2021)
|
| Protein yield (g/L or g/g?) |
0.107 g/L (Park et al., 2021)
|
| Temperature used in study |
23°C (Park et al., 2021)
|
| pH used in study |
pH 6.5 (Park et al., 2021)
|
| C & N source |
Glucose, galactose, yeast extract, glycerol (Park et al., 2021)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies |
NA |
| Publications/references |
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Park, S., Kang, B., Lee, H., Lee, S., Kim, H., Choi, H., Park, T. J., & Kong, K. (2021). Efficient brazzein production in yeast (Kluyveromyces lactis) using a chemically defined medium. Bioprocess and Biosystems Engineering, 44(4), 913–925. https://doi.org/10.1007/s00449-020-02499-y
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Spohner, S. C., Schaum, V., Quitmann, H., & Czermak, P. (2016). Kluyveromyces lactis: An emerging tool in biotechnology. Journal of Biotechnology, 222, 104–116. https://doi.org/10.1016/j.jbiotec.2016.02.023
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Barba, D., Beolchini, F., Del Re, G., Di Giacomo, G., & Veglió, F. (2001). Kinetic analysis of Kluyveromyces lactis fermentation on whey: batch and fed-batch operations. Process Biochemistry, 36(6), 531–536. https://doi.org/10.1016/s0032-9592(00)00242-9
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Gomes, A. M. V., Carmo, T. S., Carvalho, L. S., Bahia, F. M., & Parachin, N. S. (2018). Comparison of Yeasts as Hosts for Recombinant Protein Production. Microorganisms, 6(2), 38. https://doi.org/10.3390/microorganisms6020038
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Van Ooyen, A. J., Dekker, P., Huang, M., Olsthoorn, M. M., Jacobs, D. I., Colussi, P. A., & Taron, C. H. (2006). Heterologous protein production in the yeastKluyveromyces lactis. FEMS Yeast Research, 6(3), 381–392. https://doi.org/10.1111/j.1567-1364.2006.00049.x
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Fonseca, G. G., Heinzle, E., Wittmann, C., & Gombert, A. K. (2008). The yeast Kluyveromyces marxianus and its biotechnological potential. Applied Microbiology and Biotechnology, 79(3), 339–354. https://doi.org/10.1007/s00253-008-1458-6
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